Surgical Procedures for Melanocyte Transplant

Surgical Procedures for Melanocyte Transplant

Cellular Separation:

Well-pigmented melanocytes engender pure colonies of pigmented cells, but cells separate cell type, or premelanocyte, which can differentiate spontaneously and stably in culture; this cell type includes both unpigmented and faintly pigmented cells.

Steps for Cellular Separation:

• The epidermal layer is incubated in 0.25% trypsin EDTA, solution, in a petri dish, for 45min-60min
•  The dermal surface should be kept above
• This petridish is now placed in a Co2incubator at 370c, 5% Co2, for 50 minutes
• The epidermal sheets are gently manipulated with forceps to dissociate the epidermal cells
• Residues of dermis are discarded
• Patients serum about 0.5-1ml is added to neutralize trypsin
• The cells are washed with DMEM solution, centrifuged for 5 min at 1500rpm
• Supernant solution is discarded and the cellular pallet is mixed with patients serum (1-2ml)
• This is filled in insulin syringes, and is ready for transplant.

For more detail about this procedure, please visit - http://www.whitespotcure.com/procedures.html